For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The FRETWorks™ S•Tag™ Assay is a FRET-based method that enables extremely sensitive detection of S•Tag fusion proteins in minutes with a homogeneous format. The interaction of the 15–amino acid S•Tag fusion peptide with purified S-protein reconstitutes RNase activity, which is measured using the FRET ArUAA substrate. The substrate consists of a short, mixed ribo/deoxyribooligonucleotide having a fluorophore on the 5′-end and a quencher on the 3′-end (Keleman 1999). Fluorescence of the uncleaved molecule is inhibited by the interaction of the fluorophore and quencher; upon cleavage of the intervening ribonucleotide residue, the fluorophore is released and becomes highly fluorescent. The specificity of the substrate permits this assay to be performed with crude extracts. No reagent injection is necessary, and the signal is stable for > 6 hours.
The FRETWorks S•Tag Assay Kit is compatible with the PopCulture® Reagent (including the additives Benzonase® Nuclease and rLysozyme™ Solution), making these reagents an ideal combination for rapid, automatable screening of protein expression.
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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