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69282 Single dA™ Tailing Kit - Novagen

Addition of single 3ʹ-dA overhangs for cloning into AccepTor™ Vectors

Single dA™ Tailing Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69282
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The Single dA™ Tailing Kit is used to prepare DNA with any type of end for cloning into the Novagen AccepTor™ Vectors. The kit provides the reagents needed to blunt DNA ends and add a single 3′-dA residue. This treatment creates vector-compatible overhangs and effectively prevents insert-to-insert ligation, thus eliminating tandem inserts. The positive control included in the kit is the blunt-ended HSV•Tag® 36-mer that encodes the 11-aa peptide recognized by the HSV•Tag Monoclonal Antibody.

      Applications

      • Treatment of PCR products of proofreading DNA polymerases for cloning into AccepTor Vectors (alternative to blunt-end cloning)
      • Treatment of randomly cut or sheared DNA to be cloned into dU- or dT-tailed vectors
      Catalogue Number69282
      Brand Family Novagen®
      References
      Product Information
      Components
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69282 0

      Documentation

      Citations

      Title
    • C. Hatfield, et al. (1997) Epitope mapping by recombination PCR mutagenesis. Bio/Techniques 22, 332-337.
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      User Protocols

      Title
      TB059 Single dA™ Tailing Kit