70976
SpinPrep™ PCR Clean-up Kit
Rapid purification of PCR products for downstream procedures
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The SpinPrep™ PCR Clean-Up Kit is designed
for the rapid purification of DNA amplified
in PCR. The 10-minute procedure involves
addition of a binding buffer followed by
adsorption of the DNA to a silica membrane in
a spin column format. Following a wash step,
the DNA is eluted in low-salt buffer. This kit
removes DNA polymerases, dNTPs, salts, and
> 99% of primers so that they do not interfere
with downstream applications such as cloning,
sequencing, or labeling. PCR products from
100 bp to > 12,000 bp can be cleaned up, with
recoveries of amplified DNA in the range of
60-90% under standard conditions.
| Column binding capacity: | up to 6 µg |
| PCR volume: | 100 µl/rxn |
| Typical recovery: | 60–90% |
| Size range: | 100 bp to > 12,000 bp |
| Time required: | < 10 min |
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| Storage | +15°C to +30°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS |
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