For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
| Related products | |
|---|---|
| 69672 | Biotinylated Thrombin |
| Related products for Thrombin, Restriction Grade | |
| 69672 | Biotinylated Thrombin |
| Product information | |||||||||||||||
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| Molar mass | 37,000 | ||||||||||||||
| Avoid freeze/thaw | Yes | ||||||||||||||
| Unit definition | One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl2, 50 µg fusion protein. | ||||||||||||||
| Components |
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| Source | Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. | ||||||||||||||
| RTECS | XO8950000 | ||||||||||||||
| CAS number | 9002-04-4 | ||||||||||||||
| Store and ship information | |||
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| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |
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