How can I isolate and purify a molecule from my existing feed stream without damaging it?
We’d like to isolate additional molecules from our existing feed stream. We’ve been unsuccessful in designing a process that would achieve the correct concentration without damaging the molecules or requiring additional capital for our equipment. How can we minimize the shear stress caused by multiple pump passes and decrease process volume prior to the chromatography step?
Our Solution:We recommended that the customer use Single-Pass Tangential Flow Filtration (TFF) for this process, as we believed it would meet their high concentration goals, while minimizing stress placed on the molecules. We ran bench-scale trials to determine the proper membrane, number of sections, processing conditions and membrane area. This was followed by a pilot scale run to confirm process conditions and make larger quantities of materials for process validation. The trials identified Pellicon® 3 Cassettes with Biomax® Membrane as the best option for recovery and purity of the customer’s molecules.
Customer Outcome:The customer ultimately selected our Single-Pass TFF option including Pellicon® 3 cassettes with Biomax® 50 membrane due to its simplicity and effective performance, as well as successful scale-up capabilities. Additionally, they appreciated the ongoing on-site and off-site support available to them throughout the evaluation and implementation process. A successful outcome was possible thanks to the approach and capabilities of Single-Pass TFF which helped to reduce process volume, eliminating the need for additional capital investment for their chromatography step.
Watch these webinars for more information:
- Single-Pass Tangential Flow Filtration: A Critical Operation Within the Continuous Bioprocess
- High Viscosity Ultrafiltration Formulation for Plasma IgG and mAbs
- Application of QbD Principles to Ultrafiltration Operations in Bioprocessing
Learn more about Single-Pass TFF technology:
How can I optimize a new chromatography process efficiently and quickly?
We’re developing the chromatography step for a new process and our flow rates and purity levels are much lower than our targets. We’re under pressure to get this process validated quickly but we have limited resources to make the necessary improvements. How can we optimize this chromatography step efficiently and quickly?