Bioprocessing Cell Lines
A cell line that maintains favorable growth and productivity characteristics is vital for biopharmaceutical manufacturing processes, whether you are producing monoclonal antibodies, recombinant proteins, or viral vectors for gene therapies. Our ready-to-use cell line systems and platforms can accelerate your upstream development and greatly reduce the time, cost, and risk of developing new cell lines. Our cell lines are rationally engineered and designed for compliant biomanufacturing.
Key features include:
- Fully traceable cell lines with comprehensive documentation
- cGMP banked and characterized
- Paired, optimized, and chemically defined media and feeds
- Comprehensive user protocols for efficient cell line development and seamless scale-up, supported by technical consultation
Related Resources
- Data Sheet: CHOZN® Elite Cell Line Product Information Sheet
CHOZN® Elite: : A production adapted host cell line to fulfill all your bioprocessing needs.
- Flyer: Sf-RVN® Platform
With the Sf-RVN® Platform, we provide a rhabdovirus-free cell line that improves the safety profile of your bioprocesses combined with a chemically defined medium specifically formulated to get excellent growth and productivity.
- Product Information Sheet: Sf-RVN® Insect Cell Line
Sf-RVN® Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP).
- Product Information Sheet: EX-CELL® CD Insect Cell Medium
The EX-CELL® CD Insect Cell Medium is a specialized medium with a defined chemical composition, engineered to maximize the performance of insect cells. The formulation is animal-component free and has been formulated with L-Glutamine.
- White Paper: Development of a Novel Sf9 Rhabdovirus-Negative Cell Line (Sf-RVN®) and Companion Chemically Defined Medium
This whitepaper describes the development of a novel Sf9 Rhabdovirus-negative cell line, named Sf-RVN®, with companion chemically defined medium, the EXCELL® CD Insect Cell Medium. Combined, these two products form the Sf-RVN® Platform and offer a SfRhabdovirus free alternative for the BICS production of biologics.
- White Paper: Considerations for Bioreactor Process Development and Scale-up for Transient Transfection-based Lentivirus Production in Suspension
This white paper describes considerations for suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
- Tool: CHOZN® Omics Explorer
- White Paper: A Multifaceted Strategy for Viral Safety in AAV Processes
This white paper describes an approach to adventitious viral risk mitigation which encompasses this multifaceted approach defined as “prevent, detect, remove”.
- Brochure: VirusExpress® 293 Production Platform
Draw on our experience to get on the fast track through production.
- Data Sheet: VirusExpress® AAV Production Platform
The VirusExpress® Platform offers a transfectionbased solution to Adeno-Associated Viral (AAV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and a clinically relevant process dramatically reducing time to commercial production.
- Data Sheet: VirusExpress® Lentivirus Production Platform
The VirusExpress® Platform offers a transfection-based solution to lentiviral (LV) production challenges, featuring a suspension adapted cell line, chemically defined medium, and process with proven performance at 50 L scale.
Chozn® Cell Lines for Production of Biologics and Recombinant Proteins
CHOZN® GS-/- Cell Line, A ZFN-Modified CHO Cell Line
Powered by CompoZr® Zinc Finger Nuclease (ZFN) technology, the well-known and widely adopted CHOZN® GS-/- cell line enables rapid cell line development by metabolic selection without the addition of MSX (GS inhibitor). With excellent stability, more than 75% of clones maintain higher than 70% titer over 60 generations.
The cell line is originated from ECACC CHO K1, adapted to suspension growth in chemically defined, animal component-free media. Optimum performance is achieved when the cell line is paired with chemically defined cell culture medium and feeds.
For more information on the CHOZN® GS-/- cell line, please explore here.
CHOZN® Elite cell line, the next generation CHO host to boost your yield
The CHOZN® Elite cell line is a subclone of the CHOZN® GS-/-cell line that outperforms the parental cell line in cell line development (CLD) efficiency, predictable growth, protein production, and other performance metrics. This cell line is fully traceable from CHOZN® GS-/- ZFN modified CHO cell line and is cGMP banked, characterized and adventitious agent tested to meet regulatory expectations.
- High producing clones: achieves titers of 7-13 g/L with standard vectors, resulting in cost savings
- Highly stability: 85% of clones maintain higher than 70% of titer over 60 generations
- Consistent growth profile: 18 to 24 x 106 cells/mL for fed-batch
- Easy to adopt: plug-and-play with your established CLD processes, for use in both fed-batch and perfusion processes.
For more information on the CHOZN® Elite cell line, please explore here.
Cell Lines for Recombinent Proteins, Virus Like Particles and Viral Vector Production
Sf-RVN® Platform For Improving the Safety Profile of Your Bioprocesses
The Sf-RVN® Insect Cell Line with companion EX-CELL® CD Insect Cell Medium provides a high performing platform optimized for production of recombinant proteins, viral vaccines such as viral like particles (VLP) and adeno-associated vectors (AAV) for gene therapy applications.
The majority of Spodoptera frugiperda cells (such as Sf-9) contain a rhabdovirus that is considered a process contaminant and must be eliminated during bioprocessing.
The Sf-RVN® Platform is a synergy between a patented Sf9 rhabdovirus-negative cell line that demonstrates same characteristics as Sf-9 cells, with companion chemically defined medium, specifically formulated for excellent growth and productivity.
For more information on the Sf-RVN® Insect Cell Line please explore here.
VirusExpress® Lentiviral Production Platform
The VirusExpress® Lentiviral Platform is a transfection-based solution to lentiviral production challenges and offers a scalable upstream process optimized for suspension-based lentivirus production. The platform saves time in process development and scale-up and can be used to accelerate delivery of your therapy to patients.
Key benefits of HEK293T VirusExpress® Cells include:
- Cell line grows to high cell density and viability in suspension and has low doubling time
- Selected for higher lentivirus production using PEI-based transient transfection method
- GMP-banked, fully characterized and adventitious agent tested
- Licenses available for research or clinical/commercial use.
- Fully traceable and documentation available for regulatory filings
For additional information on VirusExpress® Lentiviral Platform, please explore here.
VirusExpress® AAV Production Platform
The VirusExpress® AAV Production Platform offers a transfection-based solution to Adeno-Associated Viral (AAV) production challenges, and an upstream process optimized for scalable, suspension-based AAV production. The platform saves time in process development and scale-up and can be used to accelerate delivery of your therapy to patients.
Key benefits of HEK293 VirusExpress® Cells
- Cell line grows to high cell density and viability in suspension and has low doubling time
- Selected for higher AAV production using PEI-based transient transfection method
- GMP-banked, fully characterized and adventitious agent tested
- Licenses available for research or clinical/commercial use
- Fully traceable and documentation available for regulatory filings
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