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MABT1340-25UG Anti-Lamin A/C Antibody, clone 2A1

Anti-Lamin A/C, clone 2A1, Cat. No. MABT1340, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Lamin A/C Antibody, clone 2A1 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: LMNA, LMN1 UniProt Number: P02545
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MABT1340-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ht, MkICC, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1340-25UG
      DescriptionAnti-Lamin A/C Antibody, clone 2A1
      Alternate Names
      • 70 kDa Lamin
      • Renal carcinoma antigen NY-REN-32
      Background InformationPrelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32). Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Lamin A/C, clone 2A1, Cat. No. MABT1340, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative immunoprecipitated Lamin A/C in culture supernatant (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).


      Western Blotting Analysis: A representative lot detected Lamin A/C in culture supernatants of various cell lines (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).


      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.


      Immunofluorescence Analysis: A representative lot detected Lamin A/C in the nuclear interior of HeLa cells (Data courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenPurified protein corresponding to the rod domain for human Lamin A/C.
      Clone2A1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2A1 detects the Lamin A/C species in the nuclear interior in multiple species. It targets the Rod domain of Lamin A/C.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Hamster
      • Monkey
      Species Reactivity NoteHuman, Hamster, Monkey, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LMNA
      • LMN1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight74 and 65 kDa observed. 74.14 kDa and 65.14 kDa for Lamin A and C. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected Lamin A/C in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT1340-25UG 04054839573125