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ABC468 Anti-MAGEA3 Antibody/MAGE3

Anti-MAGEA3 Antibody/MAGE3 is an antibody against MAGEA3/MAGE3 for use in western blotting & IHC (Paraffin).

Anti-MAGEA3 Antibody/MAGE3: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MAGEA3, MAGE3 UniProt Number: P43357
ABC468
100 µL  
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      Übersicht

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC468
      DescriptionAnti-MAGEA3 Antibody/MAGE3
      Alternate Names
      • Melanoma-associated antigen 3
      • Antigen MZ2-D
      • Cancer/testis antigen 1.3
      • CT1.3
      • MAGE-3 antigen
      Background InformationMAGEA3/MAGE3, also known as Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen, and encoded by the gene MAGEA3/MAGE3, is an interesting protein with multiple roles. MAGEA3/MAGE3 is primarily an accessory protein that assists in ubiquitin ligase activity of E3 ubiquitin protein ligases. MAGEA3/MAGE3 enhances the ligase activity of TRIM28, but also acts to stabilize various E2 conjugating ubiquitin enzymes with the E3 target protein. Developmentally, MAGEA3/MAGE3 plays a role in growth promotion, and hence, it also plays a role in tumor transformation and tumor progression. In melanoma it enhances viability and is an antigen for cytolytic T lymphocytes. MAGEA3/MAGE3 expression is limited to early developmental stages normally, but it is found expressed in many kinds of cancers including breast, lung, melanoma, and head and neck cancers. Because MAGEA3/MAGE3 can serve as a T cell antigen in melanoma, various research groups are using MAGEA3/MAGE3 in attempts to create vaccines and other immune treatments for melanoma, and work is underway to understand how to manage such treatments in patients with the disease.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MAGEA3 Antibody/MAGE3 is an antibody against MAGEA3/MAGE3 for use in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected MAGEA3/MAGE3 in 10 µg of MDA-MB-231, MDA-MB-435, MCF10A, MDA-MB-468, T47D, and HCC1143 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MAGEA3/MAGE3 in human breast cancer and human testis tissue.
      Biological Information
      ImmunogenBlue carrier protein-conjugated linear peptide corresponding to human MAGEA3/MAGE3 near the C-terminus.
      EpitopeNear C-terminus
      Concentration0.5 mg/mL
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Immunogen is 73% identical in mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAGEA3
      • MAGE3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~47 kDa observed. The calculated molecular weight is 35 kDa, however MAGEA3/MAGE3 has been shown as a ~49 kDa band in western blots (Kocher, T., et al. (1995). Cancer Research. 55:2236-2239).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected MAGEA3/MAGE3 in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABC468 04053252977510

      Documentation

      Anti-MAGEA3 Antibody/MAGE3 Analysenzertifikate

      TitelChargennummer
      Anti-MAGEA3/MAGE3 - Q2432702 Q2432702
      Anti-MAGEA3/MAGE3 -2700260 2700260

      Literatur

      ÜbersichtPub Med ID
      Identification and intracellular location of MAGE-3 gene product.
      Kocher, T, et al.
      Cancer Res., 55: 2236-9 (1995)  1994

      Abstract anzeigen       		7757970 7757970

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