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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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ABT13
Sigma-AldrichAnti-Sarcolipin Antibody
Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.
More>>Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting. Less<<
Anti-Sarcolipin Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Sarcolipin (UniProt: O00631; also known as SLN) is encoded by the SLN gene (Gene ID: 6588) in human. Sarcolipin is a single-pass membrane protein that contains a cytoplasmic domain (aa 1-7), a transmembrane domain (aa 8-26), and a lumenal domain (aa 27-31). SLN is a regulator of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in muscle and plays a role in regulating muscle thermogenesis and metabolism. It increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in muscle and contributes to non-shivering thermogenesis. This heat generating process is mechanistically distinct from and unrelated to Ca2+-linked thermogenic processes in skeletal muscle. It acts by reducing the apparent affinity of the ATPase for Ca2+. SLN is also reported to modulate calcium re-uptake during muscle relaxation and play an important role in calcium homeostasis in muscle. Higher levels of SLN have been reported in patients with Duchenne muscular dystrophy and reducing SLN levels improves dystrophic pathology. (Ref.: Campbell, KL., and Dicke, AA (2018). Front. Physiol. 9; Article 714; Voit, A., et al. (2017). Nat. Commun. 8; Article 1068).
References
Product Information
Format
Affinity Purified
HS Code
3002 15 90
Control
Rabbit quadraceps tissue
Presentation
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Peptide Inhibition Assay: Sarcolipin detection in Rabbit Quadricept tissue lysate was completely inhibited by preblocking of this antibody with 1 mg of antigen peptide for 1 hour at 37⁰C .
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Sarcolipin in multiple mammalian species. It targets an epitope within 6 amino acids from the C-terminal lumenal domain.
Species Reactivity
Rabbit
Species Reactivity Note
Rabbit. Predicted to react with Bovine, Human, Mouse, Monkey, Rat based on 100% sequence homology.
Sarcoplasmic reticulum Ca(2+)-ATPases are transmembrane proteins that catalyze the ATP-dependent transport of Ca(2+) from the cytosol into the lumen of the sarcoplasmic reticulum in muscle cells. This gene encodes a small proteolipid that regulates several sarcoplasmic reticulum Ca(2+)-ATPases. The transmembrane protein interacts with Ca(2+)-ATPases and reduces the accumulation of Ca(2+) in the sarcoplasmic reticulum without affecting the rate of ATP hydrolysis.
SEQUENCE SIMILARITIES: Belongs to the sarcolipin family.
Molecular Weight
~6 kDa observed; 3.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Rabbit Quadriceps muscle tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Sarcolipin in Rabbit Quadriceps muscle tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
