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ABC505 Anti-hERV-WL Antibody

Detect HERV using this rabbit polyclonal antibody, Anti-hERV-WL Antibody validated for use in western blotting & Flow Cytometry.

Anti-hERV-WL Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Sec23b UniProt Number: P61561
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ABC505
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABC505
      DescriptionAnti-hERV-WL Antibody
      Alternate Names
      • rWL-1
      • Protein transport protein Sec23B
      • SEC23-related protein B
      Background InformationHuman endogenous retrovirus (HERV) proviruses comprise a significant part of the human genome, with approximately 98,000 ERV elements and fragments making up nearly 8% of the DNA. All appear to be inactive containing many deletions and nonsense mutations having integrated into the genome likely millions of years ago but left over HERV DNA as played an active role in genome evolution for millions and years and many important genes expressed today have promoters and enhances that have come from HERV DNA sequences. The best studied example is that of the retroviral fusogenic env proteins. In humans, and other mammals, intact env proteins called syncytins are responsible for the formation and function of syncytiotrophoblasts. These multi-nucleated cells are mainly responsible for maintaining nutrient exchange and protecting the developing fetus from the mother's immune system. The protein HERV-WL is an ancient retroviral envelope (env) protein. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. HERV-WL gets cleaved into two functional chains upon expression. A surface protein named SU and a transmembrane protein called TM. SU mediates receptor recognition. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. The transmembrane protein (TM) acts as a class I viral fusion protein. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. HERV is expressed during development and in the uterine and placental tissues in the adult.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum in buffer containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect HERV using this rabbit polyclonal antibody, Anti-hERV-WL Antibody validated for use in western blotting & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected hERV-WL in Jurkat cell lysate.

      Flow Cytometry Analysis: 1 µL from a representative lot detected hERV-WL in 1X10E6 Raji and COS-7 cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human ERV-WL.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Sec23b
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~60 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Tera-2 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hERV-WL in 10 µg of Tera-2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABC505 04053252937477