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MABN2419-25UG Anti-phospho-alpha-synuclein (Ser129) Antibody, clone LS4-1B1

Anti-phospho-alpha-synuclein (Ser129), clone LS4-1B1, Cat. No. MABN2419, is a highly specific mouse monoclonal antibody that targets alpha-synuclein and neurofilament light polypeptide (NFL) phosphorylated on Ser129 and has been tested in Western Blotting and Immunohistochemistry..

Research Category: Neuroscience Gene Symbol: SNCA, NACP, PARK1 UniProt Number: P37840
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MABN2419-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2419-25UG
      DescriptionAnti-phospho-alpha-synuclein (Ser129) Antibody, clone LS4-1B1
      Alternate Names
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson's disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-alpha-synuclein (Ser129), clone LS4-1B1, Cat. No. MABN2419, is a highly specific mouse monoclonal antibody that targets alpha-synuclein and neurofilament light polypeptide (NFL) phosphorylated on Ser129 and has been tested in Western Blotting and Immunohistochemistry..
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Western Blotting applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

      Immunohistochemistry Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Immunohistochemistry applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).
      Biological Information
      ImmunogenKLH-conjugated linear peptides corresponding to aa 121-137 and 124-134 phosphorylated on Serine 129 from the C-terminal region of human alpha synuclein.
      CloneLS4-1B1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone LS4-1B1 specifically detects alpha-synuclein phosphorylated on serine 129.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNCA
      • NACP
      • PARK1
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed (aggregated); 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected phospho-alpha-synuclein (Ser129) in mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABN2419-25UG 04054839431883

      Documentation