Millipore Sigma Vibrant Logo

344270 Forskolin, Coleus forskohlii - CAS 66575-29-9 - Calbiochem

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: 7β-Acetoxy-8,13-epoxy-1α,6β,9α-trihydroxy-labd-14-en-11-one, Colforsin

Primary Target: Adenylate cyclase
View Products on Sigmaaldrich.com
344270
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

CAS #Empirical Formula
66575-29-9C₂₂H₃₄O₇

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
344270-10MG
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 10 mg
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      344270-25MG
      Verfügbarkeit wird abgerufen...
      —
      Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
      Lieferbar 
      Produkt wurde eingestellt
      Begrenzter Lagerbestand
      Bestätigung der Verfügbarkeit erforderlich
        Restmenge: Angebot folgt
          Restmenge: Angebot folgt
          Bitte erfragen
          Kontakt zum Kundenservice
          Contact Customer Service

          		 Kst.-Ampulle 25 mg
          Preis wird abgerufen...
          Preis nicht abrufbar
          Die Mindestmenge muss ein Vielfaches sein von
          Maximum Quantity is
          Bei Bestätigung Weitere Informationen
          Sie haben () gespart
           
          —
          Bitte erfragen
          		 —
          344270-50MG
          Verfügbarkeit wird abgerufen...
          —
          Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
          Lieferbar 
          Produkt wurde eingestellt
          Begrenzter Lagerbestand
          Bestätigung der Verfügbarkeit erforderlich
            Restmenge: Angebot folgt
              Restmenge: Angebot folgt
              Bitte erfragen
              Kontakt zum Kundenservice
              Contact Customer Service

              		 Kst.-Ampulle 50 mg
              Preis wird abgerufen...
              Preis nicht abrufbar
              Die Mindestmenge muss ein Vielfaches sein von
              Maximum Quantity is
              Bei Bestätigung Weitere Informationen
              Sie haben () gespart
               
              —
              Bitte erfragen
              		 —
              Description
              OverviewThe major cell-permeable diterpene isolated from the Indian plant Coleus forskohlii. At low doses, it acts as a positive inotropic agent. At higher doses, it serves as a hypotensive and vasodilatory agent due to its actions as a smooth muscle relaxant. No major side effects are observed at effective doses. Forskolins pharmacological activities are due to its activation of adenylate cyclase (EC50 = 4 µM), resulting in increased cAMP levels. The exact mechanism of forskolins positive inotropic effect is unknown but may be related to a cAMP-dependent increase in Na+ permeability that results in indirect augmentation of Ca2+ release. Inhibits MAP kinase in rat renal mesangial cells (IC50 = 25 µM). Also acts as a Hh pathway antagonist. Shown to inhibit apoptosis in cerebellar granule cells and to induce apoptosis in resting human B lymphocytes.
              Catalogue Number344270
              Brand Family Calbiochem®
              Synonyms7β-Acetoxy-8,13-epoxy-1α,6β,9α-trihydroxy-labd-14-en-11-one, Colforsin
              References
              ReferencesD'Orazio, J.A., et al. 2006. Nature 443, 340.
              Noveen, A., et al. 1996. Biochem. Biophys. Res. Commun. 219, 180.
              Galli, C., et al. 1995. J. Neurosci. 15, 1172.
              Li, X., et al. 1995. Am. J. Physiol. 269, C986.
              Lomo, J., et al. 1995. J. Immunol. 154, 1634.
              Uneyama, H., et al. 1993. J. Biol. Chem. 268, 168.
              Laurenza, A., et al. 1989. Trends Pharmacol. Sci. 10, 442.
              Adashi, E.Y., and Resnick, C.E. 1986. J. Cell. Biochem. 31, 217.
              Seamon, K.B., and Daly, J.W. 1986. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20, 1.
              Huang, R., et al. 1982. Cyclic Nucleotide Res. 8, 385.
              Metzger, H., and Lindner, E. 1981. IRCS Med. Sci. Biochem. Cardiovasc. System Pharmacol. 9, 99.
              Product Information
              CAS number66575-29-9
              ATP CompetitiveN
              FormWhite to off-white crystalline solid
              Hill FormulaC₂₂H₃₄O₇
              Chemical formulaC₂₂H₃₄O₇
              HS Code2932 99 99
              ReversibleN
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Primary TargetAdenylate cyclase
              Primary Target IC<sub>50</sub>25 µM inhibiting MAP kinase in rat renal mesangial cells; EC50 = 4 µM for activation of adenylate cyclase
              Purity≥99% by HPLC
              Physicochemical Information
              Cell permeableY
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSQL6150000
              Safety Information
              R PhraseR: 21

              Harmful in contact with skin.
              S PhraseS: 36

              Wear suitable protective clothing.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). DMSO stock solutions are stable for up to 4 months at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Bestellnummer GTIN
              344270-10MG 07790788049294
              344270-25MG 04055977215359
              344270-50MG 04055977215366

              Documentation

              Forskolin, Coleus forskohlii - CAS 66575-29-9 - Calbiochem SDB

              Titel

              Sicherheitsdatenblatt (SDB) 

              Forskolin, Coleus forskohlii - CAS 66575-29-9 - Calbiochem Analysenzertifikate

              TitelChargennummer
              344270

              Literatur

              Übersicht
              D'Orazio, J.A., et al. 2006. Nature 443, 340.
              Noveen, A., et al. 1996. Biochem. Biophys. Res. Commun. 219, 180.
              Galli, C., et al. 1995. J. Neurosci. 15, 1172.
              Li, X., et al. 1995. Am. J. Physiol. 269, C986.
              Lomo, J., et al. 1995. J. Immunol. 154, 1634.
              Uneyama, H., et al. 1993. J. Biol. Chem. 268, 168.
              Laurenza, A., et al. 1989. Trends Pharmacol. Sci. 10, 442.
              Adashi, E.Y., and Resnick, C.E. 1986. J. Cell. Biochem. 31, 217.
              Seamon, K.B., and Daly, J.W. 1986. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20, 1.
              Huang, R., et al. 1982. Cyclic Nucleotide Res. 8, 385.
              Metzger, H., and Lindner, E. 1981. IRCS Med. Sci. Biochem. Cardiovasc. System Pharmacol. 9, 99.

              Broschüre

              Titel
              Activators and Inhibitors of Adenylate Cyclase Technical Bulletin

              Literaturstellen

              Titel
            • Ryan W Carlin, et al. (2006) PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3- transporters. American Journal of Physiology Cell Physiology 290, C1560-C1571.
            • Iwona Bogacka, et al. (2005) Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro. Journal of Clinical Endocrinology and Metabolism in press,.
            • Michael J. Cross, et al. (2002) The Shb Adaptor Protein Binds to Tyrosine 766 in the FGFR-1 and Regulates the Ras/MEK/MAPK Pathway via FRS2 Phosphorylation in Endothelial Cells. Molecular Biology of the Cell 13, 2881-2893.
              		•
              Datenblatt

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision20-November-2008 JSW
              Synonyms7β-Acetoxy-8,13-epoxy-1α,6β,9α-trihydroxy-labd-14-en-11-one, Colforsin
              DescriptionA cardioactive diterpene isolated from the Indian plant Coleus forskolii. At low doses, acts as a positive inotropic agent in dogs, cats, spontaneously hypertensive and normal rats, and isolated guinea pig heart. At higher doses, acts as a hypotensive and vasodilatory agent due to its action as a smooth muscle relaxant. No major side effects are observed at effective doses. Rapid and reversible activator of adenylate cyclase (EC50 = 4 µM) in membranes and intact cells. Does not affect the activity of guanylate cyclase or cyclic nucleotide phophodiesterases. The exact mechanism of forskolin's positive inotropic effect is unknown but may be related to a cAMP-dependent increase in Na+ permeability, which results in indirect augmentation of Ca2+ release. Inhibits MAP kinase in rat renal mesangial cells (IC50 = 25 µM). Also acts as a Hh pathway antagonist. Shown to inhibit apoptosis in cerebellar granule cells and to induce apoptosis in resting human B-lymphocytes.
              FormWhite to off-white crystalline solid
              CAS number66575-29-9
              RTECSQL6150000
              Chemical formulaC₂₂H₃₄O₇
              Structure formulaStructure formula
              Purity≥99% by HPLC
              SolubilityDMSO (5 mg/ml). Ethanol is reported to inhibit forskolin-mediated activation of adenylate cyclase. DMSO is the recommended solvent; at ≤5% DMSO final concentration, little inhibition of the adenylate cyclase activation will occur.
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). DMSO stock solutions are stable for up to 4 months at 4°C.
              Toxicity Harmful
              Merck USA index14, 2476
              ReferencesD'Orazio, J.A., et al. 2006. Nature 443, 340.
              Noveen, A., et al. 1996. Biochem. Biophys. Res. Commun. 219, 180.
              Galli, C., et al. 1995. J. Neurosci. 15, 1172.
              Li, X., et al. 1995. Am. J. Physiol. 269, C986.
              Lomo, J., et al. 1995. J. Immunol. 154, 1634.
              Uneyama, H., et al. 1993. J. Biol. Chem. 268, 168.
              Laurenza, A., et al. 1989. Trends Pharmacol. Sci. 10, 442.
              Adashi, E.Y., and Resnick, C.E. 1986. J. Cell. Biochem. 31, 217.
              Seamon, K.B., and Daly, J.W. 1986. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20, 1.
              Huang, R., et al. 1982. Cyclic Nucleotide Res. 8, 385.
              Metzger, H., and Lindner, E. 1981. IRCS Med. Sci. Biochem. Cardiovasc. System Pharmacol. 9, 99.
              Citation
            • Ryan W Carlin, et al. (2006) PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3- transporters. American Journal of Physiology Cell Physiology 290, C1560-C1571.
            • Iwona Bogacka, et al. (2005) Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro. Journal of Clinical Endocrinology and Metabolism in press,.
            • Michael J. Cross, et al. (2002) The Shb Adaptor Protein Binds to Tyrosine 766 in the FGFR-1 and Regulates the Ras/MEK/MAPK Pathway via FRS2 Phosphorylation in Endothelial Cells. Molecular Biology of the Cell 13, 2881-2893.
              		•