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374805 Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: KLH

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      Übersicht

      Replacement Information
      Description
      OverviewStable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
      Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
      Catalogue Number374805
      Brand Family Calbiochem®
      SynonymsKLH
      References
      ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
      Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
      Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
      Product Information
      CAS number9013-72-3
      FormBlue to blue-gray lyophilized solid
      FormulationLyophilized from BES buffer, MgSO₄.
      PreservativeNone
      Quality LevelMQ200
      Applications
      ApplicationHemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
      Application CommentsProtein concentration: ≥60%
      Copper: ≥0.09%
      Copper to Protein ratio: ≥1.5 x 10-3
      Biological Information
      Purity≥90% by size exclusion chromatography
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      374805 0

      Documentation

      Hemocyanin, Keyhole Limpet, Megathura crenulata SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Hemocyanin, Keyhole Limpet, Megathura crenulata Analysenzertifikate

      TitelChargennummer
      374805

      Literatur

      Übersicht
      Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
      Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
      Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-March-2011 JSW
      SynonymsKLH
      DescriptionHemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
      BackgroundKeyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
      FormBlue to blue-gray lyophilized solid
      FormulationLyophilized from BES buffer, MgSO₄.
      CAS number9013-72-3
      Purity≥90% by size exclusion chromatography
      PreservativeNone
      CommentsProtein concentration: ≥60%
      Copper: ≥0.09%
      Copper to Protein ratio: ≥1.5 x 10-3
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
      Toxicity Standard Handling
      Merck USA index14, 4647
      ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
      Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
      Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

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      Kategorien

      Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Adjuvants & Immunization Tools