Millipore Sigma Vibrant Logo

PS1008 PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

This PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).

PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1

View Products on Sigmaaldrich.com
PS1008
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ch, H, MRbPolyclonal Antibody

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
PS1008-100UL
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 100 ul
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      Description
      OverviewRecognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
      Catalogue NumberPS1008
      Brand Family Calbiochem®
      SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
      Application Data
      Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
      References
      ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
      Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
      Preservative≤0.1 sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PS1008-100UL 04055977227079

      Documentation

      PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      PS1008

      Literatur

      Übersicht
      Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
      ApplicationImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application Data
      Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
      BackgroundFocal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that plays a key role in maintaining focal adhesion function and cell survival. It is phosphorylated following growth factor or integrin-mediated cell adhesion stimulation. FAK is localized to focal adhesions through its C-terminal focal adhesion targeting (FAT) domain that acts by binding to paxillin and talin that are associated with the cytoplasmic tails of integrins. Phosphorylation of FAK at Tyr861 by Src promotes this interaction between FAK and integrins.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
      IsotypeIgG
      Specieschicken, human, mouse
      Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
      Preservative≤0.1 sodium azide
      CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
      Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
      Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
      Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
      Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
      Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
      Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
      Nakamura, K., et al. 2001. Oncogene 20, 2626.
      Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
      Slack, J.K., et al. 2001. Oncogene 20, 1152.
      Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.