05-1478
Anti-RUNX2 Antibody, clone AS110
clone AS110, from mouse
Synonyme(s) :
Acute myeloid leukemia 3 protein, CBF-alpha 1, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, PEA2-alpha A, PEBP2-alpha A, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AS110, monoclonal
Application:
ICC, WB
Citations:
5
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AS110, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RUNX2(860)
General description
54-57 kDa
RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein. As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis. RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity.
Immunogen
Epitope: Internal
GST-coupled recombinant protein corresponding to amino acids 311-415 of human RUNX2.
Application
Anti-RUNX2 Antibody, clone AS110 detects level of RUNX2 & has been published & validated for use in WB & IC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
Detects RUNX2
Other species have not been tested.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Analysis Note
Routinely evaluated by Western Blot.
Western Blot Analysis:
Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Western Blot Analysis:
Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Wei Yu et al.
Frontiers in endocrinology, 11, 583105-583105 (2020-11-27)
Inhibition of neuropeptide Y1 receptor stimulates osteogenesis in vitro and in vivo. However, the underlying mechanisms involved in these effects remain poorly understood. Here we identify the effects of Y1 receptor deficiency on osteogenic differentiation in human bone marrow stromal
Tzong-Shi Lu et al.
Cardiovascular research, 96(3), 524-532 (2012-08-31)
Vascular calcification (VC) is a significant contributor to cardiovascular mortality in patients with chronic kidney disease (CKD) and coronary artery disease (CAD). Osteo/chondrocytic transformation and simultaneous dedifferentiation of smooth muscle cells (SMCs) are important in the pathogenesis of VC. Heat-shock
Zhe Shi et al.
International journal of nanomedicine, 15, 9337-9353 (2020-12-03)
Autologous bone grafts are the gold standard for treating bone defects. However, limited bone supply and morbidity at the donor site restrict its extensive use. Therefore, developing bone graft materials as an alternative to autologous grafts has gained considerable attention.
Zhe Shi et al.
International journal of nanomedicine, 16, 5603-5619 (2021-08-26)
Given that autologous bone graft for bone defects is limited by insufficient supply and morbidity at the donor site, developing biomimetic graft materials as an alternative has gained consistent attention. However, obstacles in designing bone-mimetic materials that could integrate the
Kenneth Lim et al.
Circulation, 125(18), 2243-2255 (2012-04-12)
Klotho is known to function as a cofactor for the phosphatonin, fibroblast growth factor (FGF)-23 at the kidney. FGF-23 levels rise in chronic kidney disease (CKD) despite progression of accelerated vascular calcification. There are currently conflicting data on whether FGF-23
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 05-1478 | 04053252743986 |
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique