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Merck

06-413

Anti-NFκB p52 Antibody

Upstate®, from rabbit

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Principaux documents

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Nom du produit

Anti-NFκB p52 Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

using Protein A

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_019408

UniProt accession no.

Q00653

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NFKB1(4790)

Analysis Note

Control
Raji cell lysate, Jurkat whole cell lysate
routinely evaluated by immunoblot on RIPA lysates from Raji cells

Application

Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

Recognizes NFκB p52 and its precursor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

52 kDa, and precursor (100 kDa)
The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Immunogen

KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative.
Unpurified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

IGF-I gene transfer effects on inflammatory elements present after thermal trauma.
Mohan R K Dasu, David N Herndon, Olivera Nesic, J Regino Perez-Polo
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
T Toliver-Kinsky et al.
Journal of neurochemistry, 75(6), 2241-2251 (2000-11-18)
Anovel nuclear factor kappaB (NF-kappaB) binding site has been identified within the promoter region of the mouse gene encoding choline acetyltransferase (ChAT), the enzyme that synthesizes acetylcholine and has been implicated in the cognitive deficits associated with aging and Alzheimer's
Loss of Negative Feedback Control of Nuclear Factor-{kappa}B2 Activity in Lymphocytes Leads to Fatal Lung Inflammation.
Yang L, Cui H, Wang Z, Zhang B, Ding J, Liu L, Ding HF
The American Journal of Pathology null
Nuclear factor-kappaB dimer exchange promotes a p21(waf1/cip1) superinduction response in human T leukemic cells.
Chang, PY; Miyamoto, S
Molecular Cancer Research null
Simona Saccani et al.
Molecular cell, 11(6), 1563-1574 (2003-06-25)
Transcription factors within a family usually share the ability to recognize similar or identical consensus sites. For example, the five mammalian NF-kappaB/Rel proteins generate more than 12 dimers recognizing 9-11 nucleotide kappaB sites. Each dimer selectively regulates a few target
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Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

"Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. The extracellular N-terminal region of TLRs recognizes specific pathogen components. At least 13 different members of TLR family have been identified that detect different pathogen associated molecular patterns (PAMPs), including lipopolysaccharides, flagellin, bacterial CpG DNA, and viral RNA and DNA. Recognition of PAMPs by TLRs is considered as a key process for the induction of an inflammatory response."

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Numéro d'article de commerce international

RéférenceGTIN
06-41304053252369032

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