126579
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
Synonyme(s) :
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
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About This Item
description
Merck USA index - 14, 8468
assay
≥95% (Albumin, electrophoresis)
form
lyophilized powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
immunofluorescence: suitable
impurities
≤0.2% fatty acids
≤3.0% Ash
≤5% moisture
color
pale green
solubility
aqueous buffer: 20 mg/mL
water: 20 mg/mL
shipped in
ambient
storage temp.
2-8°C
Quality Level
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Disclaimer
Toxicity: Standard Handling (A)
General description
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Material may take up to 24 h to fully dissolve.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Ravi Kumar Murumalla et al.
Lipids in health and disease, 11, 175-175 (2012-12-25)
On the basis that high fat diet induces inflammation in adipose tissue, we wanted to test the effect of dietary saturated and polysunsaturated fatty acids on human adipose tissue and adipocytes inflammation. Moreover we wanted to determine if TLR2 and
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Microvascular research, 133, 104098-104098 (2020-10-20)
During diabetes mellitus, advanced glycation end-products (AGEs) are major contributors to the development of alterations in cerebral capillaries, leading to the disruption of the blood-brain barrier (BBB). Consequently, this is often associated with an amplified oxidative stress response in microvascular
Dan Ye et al.
Structure (London, England : 1993), 26(11), 1513-1521 (2018-09-18)
We introduce resonant soft X-ray scattering (RSoXS) as an approach to study the structure of proteins and other biological molecules in solution. Scattering contrast calculations suggest that RSoXS has comparable or even higher sensitivity than hard X-ray scattering because of
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Kathryn J Newhall et al.
Clinical chemistry, 56(9), 1483-1491 (2010-07-29)
Biomarker assays are often conducted on whole blood samples in the course of drug development studies. Because bacterial lipopolysaccharide (LPS) (endotoxin) contamination is known to cause spontaneous cytokine production by monocytes, contamination of blood collection tubes may interfere with biomarker
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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