Accéder au contenu
Merck

14-440-M

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

PKA Protein, Recombinant, 10 µg, Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

biological source

human

recombinant

expressed in E. coli
expressed in BL21 DE3 cells

product line

Upstate®

form

liquid

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
8580 U/mg

shelf life

1 yr

mol wt

Mw 40.7 kDa

purified by

chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

accession no.

 (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. )
NM_002730.3

NCBI accession no.

NM_002730.3
NM_207518.1

UniProt accession no.

P17612

storage temp.

−70°C

Quality Level

100

Gene Information

human ... PRKACA(46909581)

Analysis Note

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Biochem/physiol Actions

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.
PKA
Recombinant human full length PKA, catalytic subunit type alpha, untagged

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Replaces: 14-114

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Physical form

Phosphocellulose P11 chromatography

Preparation Note

1 year at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Classe de stockage

10 - Combustible liquids

wgk

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Protein kinase A
Morrison H
Journal of Separation Science, 173-178 (2021)
PKA: a portrait of protein kinase dynamics
Taylor SS, et al.
Biochimica et Biophysica Acta, 1697(1-2), 259-269 (2004)
Protein Kinase A in cellular migration?Niche signaling of a ubiquitous kinase
Svec KV and Howe AK
Frontiers in molecular biosciences, 9 (2022)
Dana Yoo et al.
The Journal of biological chemistry, 278(25), 23066-23075 (2003-04-10)
The Kir1.1 (ROMK) subtypes of inward rectifier K+ channels mediate potassium secretion and regulate sodium chloride reabsorption in the kidney. The density of ROMK channels on the cortical collecting duct apical membrane is exquisitely regulated in concert with physiological demands.
Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Test, 200, 581-596 (1991)
Afficher tous les articles scientifiques apparentés

Numéro d'article de commerce international

RéférenceGTIN
14-44004053252588150

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique