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Merck

1.50447

SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonyme(s) :

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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A propos de cet article

UNSPSC Code:
41115709
NACRES:
NB.21
Particle size:
3.5 μm
Matrix active group:
ZIC-HILIC bonding phase
Pore size:
200 Å pore size
Matrix:
fully porous particle

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Nom du produit

SeQuant® ZIC-HILIC™ 3.5µm,200Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

agency

suitable for USP L114

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

3.5 μm

pore size

200 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

Quality Level

100

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Catégories apparentées

General description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these

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