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Nom du produit
ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set, from rabbit, purified by using Protein A
biological source
rabbit
clone
polyclonal
purified by
using Protein A
species reactivity
human, mouse
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
human ... H3F3B(3021)
Analysis Note
μ®
μμ À conserver à -20 °C.
Amorce sens :
GAT GGT TCA ACA CTC TTA CA
Application
μ
μ
Épigénétique et fonction nucléaire
Histones
Biochem/physiol Actions
Disclaimer
General description
Chaque jeu d'anticorps ChIPAb+ comprend des amorces témoins (testées sur chaque lot par PCR quantitative) vous permettant de valider biologiquement vos résultats d'immunoprécipitation dans un contexte locus-spécifique.
The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 139 bp region of human alpha-Satellite. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys27)-associated chromatin.
Immunogen
Other Notes
Packaging
Physical form
μμ °
μμ °
GAT GGT TCA ACA CTC TTA CA
Preparation Note
Legal Information
Classe de stockage
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
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Contenu apparenté
Technical Guide to Chromatin Immunoprecipitation: Critical Factors for Success
Genome-wide mapping of DNA-protein interactions and epigenetic marks using chromatin immunoprecipitation combined with next generation sequencing (ChIP-Seq) is indispensable for many gene regulation studies. The Magna ChIPSeq Kit simplifies this technique, enabling the performance of ChIP-Seq by virtually any laboratory.
Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 17-622 | 04053252328732 |
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