324405
Anti-Dopamine D4 Receptor Rabbit pAb
lyophilized, Calbiochem®
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A propos de cet article
NACRES:
NA.43
UNSPSC Code:
12352203
Species reactivity:
human, rat
Clone:
polyclonal
Application:
—
Citations:
3
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Laissez-nous vous aiderspecies reactivity
human, rat
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA
Immunoblotting (1:500)
Immunohistochemistry (1:10,000))
isotype
IgG
shipped in
ambient
Quality Level
Gene Information
human ... DRD4(1815)
rat ... Drd4(25432)
target post-translational modification
unmodified
General description
Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
Immunogen
Human
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
Application
ELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Alberto Ricci et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 50(8), 1091-1096 (2002-07-23)
Dopamine D4 receptors mediate inhibition of vasopressin-dependent sodium reabsorption by dopamine in collecting tubules. At present, the distribution of D4 receptors in other renal districts remains an open issue. The renal distribution of D4 receptor was assessed in normally innervated
Satoko Oda et al.
Frontiers in neuroanatomy, 17, 1111008-1111008 (2023-03-04)
Pial astrocytes, a cellular component of the cerebral cortex surface structure, are observed in a wide range of mammalian species. Despite being recognized as such, the functional potential of pial astrocytes has long been overlooked. Our previous research demonstrated that
Dana C Borcherding et al.
PloS one, 6(9), e25537-e25537 (2011-10-04)
Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective
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