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Merck

393206

Anti-HNE Fluorophore Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Nom du produit

Anti-HNE Fluorophore Rabbit pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Quality Level

100

Immunogen

a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH

Application

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:5000)

Immunohistochemistry (1:100-1:500)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.

Other Notes

Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.

Physical form

Serum diluted 1:1 with glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

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Ernst-Bernhard Kayser et al.
PloS one, 11(1), e0148219-e0148219 (2016-01-30)
Lack of NDUFS4, a subunit of mitochondrial complex I (NADH:ubiquinone oxidoreductase), causes Leigh syndrome (LS), a progressive encephalomyopathy. Knocking out Ndufs4, either systemically or in brain only, elicits LS in mice. In patients as well as in KO mice distinct
Erik P Rader et al.
Rejuvenation research, 20(2), 93-102 (2016-07-06)
Exercise is the most accessible, efficacious, and multifactorial intervention to improve health and treat chronic disease. High-intensity resistance exercise, in particular, also maximizes skeletal muscle size and strength-outcomes crucial at advanced age. However, such training is capable of inducing muscle
Mara Laslo et al.
Age (Dordrecht, Netherlands), 35(4), 1117-1132 (2012-05-29)
Superoxide dismutase 1 (SOD1), a critical enzyme against oxidative stress, is implicated in aging and degenerative diseases. We previously showed that a nutraceutical containing freeze-dried açai pulp promotes survival of flies fed a high-fat diet or sod1 knockdown flies fed
Olga Boyd et al.
Free radical biology & medicine, 50(11), 1669-1678 (2011-03-17)
Fruits containing high antioxidant capacities and other bioactivities are ideal for promoting longevity and health span. However, few fruits are known to improve the survival and health span in animals, let alone the underlying mechanisms. Here we investigate the effects
Yaning Sun et al.
Experimental gerontology, 50, 57-63 (2013-12-10)
Many nutraceuticals and pharmaceuticals have been shown to promote healthspan and lifespan. However, the mechanisms underlying the beneficial effects of prolongevity interventions and the time points at which interventions should be implemented to achieve beneficial effects are not well characterized.
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