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Merck

574777

Anti-Synapsin I Rabbit pAb

lyophilized, Calbiochem®

Synonyme(s) :

Rabbit Anti-Synapsin I, Synapsin I Detection Antibody

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A propos de cet article

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat, mouse, bovine
Application:
—
Citations:
20

Principaux documents

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biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human, rat, mouse, bovine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Syn1(20964)

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Catégories apparentées

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
Recognizes the synapsin I protein in rat brain.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Immunogen

Bovine
purified, brovine brain synapsin I

Application

ELISA (1:2500-1:10,000)

Frozen Sections (see application references)

Immunoblotting (1:200)

Immunocytochemistry (1:500-1:2500)

Immunoprecipitation (1 µg/200 µg lysate)

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Preparation Note

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat brain

Other Notes

Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

11 - Combustible Solids

wgk

WGK 1

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