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EZHIL8

Human IL-8 ELISA Kit

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

Synonyme(s) :

Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

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A propos de cet article

UNSPSC Code:
41116158
NACRES:
NA.84
Gene information:
human ... CXCL8(3576)
Input:
sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum
Species reactivity:
human
Storage temp.:
2-8°C
Service technique
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Nom du produit

Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

UniProt accession no.

P10145

application(s)

research use

assay range

accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%, inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CXCL8(3576)

General description

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:
  • IL-8

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Numéro d'article de commerce international

RéférenceGTIN
EZHIL8-100K5PK04065265645676
EZHIL8-100K04065265645669
EZHIL8-100K10PK04065265645683