Anti-Amyloid Precursor Protein (APP) Antibody

Epitope: C-terminus

Nine amino acid peptide from the C-terminus of APP (YKFFEQMQN)

Detect Amyloid Precursor Protein using this Anti-Amyloid Precursor Protein Antibody, C-terminus validated for use in IC, IH, IP & WB.

Immunohistochemistry: 1:100-1:400

Immunocytochemistry on NTera2 and COS cell lines: 1:100-1:400

Western blot: 1:500-1:2000: The low abundance of full length APP in untreated cells means that poor response is seen with immunoblotting of whole cell lysates. We recommend that membrane fractions be prepared to increase the loading of APP as well as remove potential interferences from soluable proteins. NTera2, Cos7 and Hela cell membrane preparations are positive by western blots.

Immunoprecipitation: 1:100-1:400.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Recognizes Amyloid Precursor Protein (APP), C-terminal. Recognizes full length APP and C-terminal fragments resulting from cleavage by secretase. May react with APLP1 and APLP2.

Serum. Liquid in PBS with 0.02% azide.

Unpurified

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Brain

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.