Accéder au contenu
Merck

AM80

Anti-PAR (Ab-1) Mouse mAb (10H)

liquid, ≥95% (SDS-PAGE), clone 10H

Synonyme(s) :

Anti-Poly (ADP-Ribose)

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
10H, monoclonal
Species reactivity:
human, Drosophila, rat, mouse
Application:
—
Citations:
31

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

10H, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, Drosophila, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (2.5 µg/mL)
Immunocytochemistry (5-20 µg/mL))

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine
purified poly(ADP-ribose)

Application

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Normal mouse IgG
Positive Control
Etoposide-treated HL-60 cells

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

J Wang et al.
Oncogene, 36(43), 5939-5947 (2017-06-27)
Understanding the mechanisms of platinum compound resistance, including cisplatin resistance, has important implications for improving cancer treatments. Previous studies identified a potential role for mitogen-activated protein kinase phosphatase-1 (MKP-1) in cisplatin resistance. This work focuses on the regulation of poly(ADP-ribose)
Dominic I James et al.
F1000Research, 5, 736-736 (2016-10-16)
After a DNA damage signal multiple polymers of ADP ribose attached to poly(ADP) ribose (PAR) polymerases (PARPs) are broken down by the enzyme poly(ADP) ribose glycohydrolase (PARG). Inhibition of PARG leads to a failure of DNA repair and small molecule
Laura Maggi et al.
European journal of immunology, 40(8), 2174-2181 (2010-05-21)
We have previously shown that human Th17 lymphocytes are characterized by the selective expression of IL-23 receptor (IL-23R), CCR6, CD161, and the transcription factor retinoic acid-related orphan receptor C (RORC), and originate from a CD161(+)CD4(+) naïve T-cell precursor in response
Li-Li Zhuang et al.
PloS one, 8(10), e77304-e77304 (2013-11-10)
All-trans retinoic acid (ATRA) is an active metabolite of Vitamin A, it shows protective effects on asthma, including maintains airway epithelial integrity, inhibits asthma effector cells differentiation, modulates immune response, et al. However, the promoting effect of ATRA on Th2
Gautier Stoll et al.
Oncoimmunology, 8(6), e1571389-e1571389 (2019-05-10)
The expression of two metabolic enzymes, i.e., aldehyde dehydrogenase 7 family, member A1 (ALDH7A1) and lipase C, hepatic type (LIPC) by malignant cells, has been measured by immunohistochemical methods in non-small cell lung carcinoma (NSCLC) biopsies, and has been attributed
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique