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Merck

MAB1281B

Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate

clone 235-1, from mouse, biotin conjugate

Synonyme(s) :

HuNu

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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Nom du produit

Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate, clone 235-1, from mouse, biotin conjugate

biological source

mouse

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Analysis Note

Control
A431 human epidermoid cancer cells.
Evaluated by Immunocytochemistry in A431 human epidermoid cancer cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in A431 human epidermoid cancer cells.

Application

Detect Nuclei Marker using this mouse monoclonal antibody, Anti-Human Nuclei Antibody, clone 235-1 & Biotin Conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs). Immunocytochemistry Analysis: A 1:200 dilution of this antibody did not detect nuclei in primary mouse embryonic fibroblasts (PMEFs).
Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neuroregenerative Medicine

Neural Stem Cell Migration

Biochem/physiol Actions

This antibody recognizes human and primate nuclei. Antibody is not reactive with rat or mouse nuclei.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.

Immunogen

Human nuclei

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Cesar A Garcia et al.
NPJ microgravity, 9(1), 92-92 (2023-12-19)
The emerging arena of space exploration has created opportunities to study cancer cell biology in the environments of microgravity and hypergravity. Studying cellular behavior in altered gravity conditions has allowed researchers to make observations of cell function that would otherwise
Ashwani Kumar Gupta et al.
Communications biology, 3(1), 231-231 (2020-05-13)
A fundamental challenge in emulating kidney tissue formation through directed differentiation of human pluripotent stem cells is that kidney development is iterative, and to reproduce the asynchronous mix of differentiation states found in the fetal kidney we combined cells differentiated
Tetsuro Tamaki et al.
Frontiers in physiology, 6, 165-165 (2015-06-18)
Skeletal muscle makes up 40-50% of body mass, and is thus considered to be a good adult stem cell source for autologous therapy. Although, several stem/progenitor cells have been fractionated from mouse skeletal muscle showing a high potential for therapeutic
Yohei Tsukada et al.
Scientific reports, 11(1), 745-745 (2021-01-14)
Angiogenesis contributes to numerous pathological conditions. Understanding the molecular mechanisms of angiogenesis will offer new therapeutic opportunities. Several experimental in vivo models that better represent the pathological conditions have been generated for this purpose in mice, but it is difficult
Kuang-Yui Chen et al.
Communications biology, 3(1), 363-363 (2020-07-11)
Histone H3.3 mutations are a hallmark of pediatric gliomas, but their core oncogenic mechanisms are not well-defined. To identify major effectors, we used CRISPR-Cas9 to introduce H3.3K27M and G34R mutations into previously H3.3-wildtype brain cells, while in parallel reverting the

Numéro d'article de commerce international

RéférenceGTIN
MAB1281B04053252917615

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