MAB1611
Anti-Keratin Epithelial Antibody, clone AE3
clone AE3, Chemicon®, from mouse
Synonyme(s) :
Type II Cytokeratin
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AE3, monoclonal
Application:
IHC, WB
Citations:
29
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified antibody
clone
AE3, monoclonal
species reactivity
rat, chicken, rabbit, mouse, human, monkey, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Immunogen
Human epidermal cells
Application
Anti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
Immunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Biochem/physiol Actions
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.
Physical form
Format: Purified
Liquid in PBS. Contains 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-588
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Classe de stockage
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms
Spagnolo, D.V. et al.
American Journal of Clinical Pathology, 84, 697-704 (1985)
Pathogenesis of fulminant monkeypox with bacterial sepsis after experimental infection with West African monkeypox virus in a cynomolgus monkey.
Nagata, N; Saijo, M; Kataoka, M; Ami, Y; Suzaki, Y; Sato, Y; Iwata-Yoshikawa, N; Ogata et al.
International Journal of Clinical and Experimental Pathology null
S R Shi et al.
The American journal of pathology, 117(1), 53-63 (1984-10-01)
Nasopharyngeal carcinoma (NPC) provides a unique opportunity to evaluate distinctive epidemiologic features and a possible etiologic relationship with Epstein-Barr virus (EBV) in human malignancy. The lack of a uniformly accepted pathologic classification for NPC has limited the application of this
H Battifora et al.
Cancer, 54(5), 843-848 (1984-09-01)
The authors investigated the presence and distribution of keratin in germ cell tumors using a rabbit-anti-keratin antiserum and a monoclonal antikeratin antibody--which is specific for keratin classes of 40, 50, and 56.5 kdaltons--by various immunohistochemical methods on frozen sections, alcohol-fixed
Viraf Sam Vasania et al.
Journal of ophthalmic & vision research, 9(4), 407-416 (2015-02-25)
To establish the efficacy and safety of ex vivo cultured autologous human conjunctival epithelial cell (hCjEC) transplantation for treatment of pterygia. Twenty-five patients with pterygia were recruited at different centers across the country. Autologous hCjEC grafts were prepared from conjunctival
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB1611 | 04053252614323 |
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