MAB3400
Anti-Vimentin Antibody
CHEMICON®, mouse monoclonal, V9
Synonyme(s) :
Vimentin antibody
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
A propos de cet article
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
V9, monoclonal
Species reactivity:
monkey, feline, pig, bovine, rat, human, chicken, canine
Application:
ICC, IF, IHC (p), WB
Citations:
90
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Anti-Vimentin Antibody, clone V9, clone V9, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
V9, monoclonal
species reactivity
monkey, feline, pig, bovine, rat, human, chicken, canine
should not react with
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
Quality Level
Gene Information
human ... VIM(7431)
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
57-60 kDa
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
Immunogen
Purified vimentin from pig eye lens
Application
Anti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB.
Immunoblotting: A dilution of 1:1000 was used in a previous lot.
Immunohistochemistry: 5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human breast tissue and patient-derived triple negative breast cancer organoid sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human liver tissue, iPSC-derived liver organoid progenitor, and mature liver organoid sections.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Vimentin in human small intestine and differentiated duodenum intestinal organoid tissue sections.
Immunocytochemistry: 5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human breast tissue and patient-derived triple negative breast cancer organoid sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human liver tissue, iPSC-derived liver organoid progenitor, and mature liver organoid sections.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Vimentin in human small intestine and differentiated duodenum intestinal organoid tissue sections.
Immunocytochemistry: 5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin
Physical form
Format: Purified
Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Analysis Note
Evaluated by Western Blot on HeLa lysates.
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3410
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Noelia López-Montero et al.
Autophagy, 12(10), 1886-1901 (2016-08-04)
Xenophagy has been studied in epithelial cells infected with Salmonella enterica serovar Typhimurium (S. Typhimurium). Distinct autophagy receptors target this pathogen to degradation after interacting with ubiquitin on the surface of cytosolic bacteria, and the phagophore- and autophagosome-associated protein MAP1LC3/LC3.
Muhammad Umar Cheema et al.
PloS one, 9(7), e101258-e101258 (2014-07-08)
Prolonged elevations of plasma aldosterone levels are associated with renal pathogenesis. We hypothesized that renal distress could be imposed by an augmented aldosterone-induced protein turnover challenging cellular protein degradation systems of the renal tubular cells. Cellular accumulation of specific protein
E Mato et al.
Journal of molecular endocrinology, 52(3), 289-300 (2014-03-20)
Tumor malignancy is associated with the epithelial-mesenchymal transition (EMT) process and resistance to chemotherapy. However, little is known about the relationship between the EMT and the multidrug-resistance gene in thyroid tumor progression. We investigated whether the expression of the ABCG2/BCRP
Subhra Prakash Hui et al.
PloS one, 10(12), e0143595-e0143595 (2015-12-03)
Zebrafish can repair their injured brain and spinal cord after injury unlike adult mammalian central nervous system. Any injury to zebrafish spinal cord would lead to increased proliferation and neurogenesis. There are presences of proliferating progenitors from which both neuronal
Shih-Pei Lin et al.
PloS one, 7(11), e49605-e49605 (2012-11-28)
Hypoxia and serum depletion are common features of solid tumors that occur upon antiangiogenesis, irradiation and chemotherapy across a wide variety of malignancies. Here we show that tumor cells expressing CD133, a marker for colorectal cancer initiating or stem cells
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB3400 | 04053252726712 |
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique