MAB3448
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
ascites fluid, clone 1MOD-1A9, Chemicon®
Synonyme(s) :
HP1b
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1MOD-1A9, monoclonal
Application:
ELISA, ICC, IHC, IP, WB
Citations:
62
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Laissez-nous vous aiderbiological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1MOD-1A9, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CBX1(10951)
General description
28 kDa
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Immunogen
Recombinant mouse HP1beta.
Application
Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
Western blot: 1:500-1:5,000
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with Heterochromatin protein-1 b (HP1b).
Analysis Note
Control
HeLa, 293 or NIH/3T3 cell lysates
HeLa, 293 or NIH/3T3 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Kyoko Hiragami-Hamada et al.
Nature communications, 7, 11310-11310 (2016-04-20)
Histone H3 trimethylation of lysine 9 (H3K9me3) and proteins of the heterochromatin protein 1 (HP1) family are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1
Sarah Herberg et al.
Scientific reports, 5, 14236-14236 (2015-09-22)
Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their
Erik Müllers et al.
Aging cell, 16(3), 575-584 (2017-03-28)
In response to DNA damage, a cell can be forced to permanently exit the cell cycle and become senescent. Senescence provides an early barrier against tumor development by preventing proliferation of cells with damaged DNA. By studying single cells, we
Mammalian ChlR1 has a role in heterochromatin organization.
Inoue, A; Hyle, J; Lechner, MS; Lahti, JM
Experimental Cell Research null
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB3448 | 04053252264900 |
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