Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5

Anti-Poliovirus 3 Antibody, clone 613-F1-B5-E5 detects level of Poliovirus 3 & has been published & validated for use in IF.

IFA at 1:100-1:400.

Final working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3.

6. Mount with buffered glycerol.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Reacts with the Poliovirus 3.

Store at -20°C for up to 12 months in convenient, undiluted aliquots. Avoid repeated freeze/thaw cycles.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.