MABS87
Anti-GPR177 Antibody, clone YJ5
clone YJ5, from mouse
Synonyme(s) :
Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, WLS, EVI
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
YJ5, monoclonal
Application:
ICC, WB
Citations:
9
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
YJ5, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... WLS(79971)
General description
Gpr177 (WLS, EVI), the mouse ortholog of Drosophila WIs, is expressed during mouse embryogenesis. It is essential for the patterning of the anterior-posterior axis during mammalian development and is activated by â-catenin and LEF/TCF-dependent transcription. Upon GPR177 activation, Wnt binds with and modifies the cellular distribution of GPR177, leading to a feedback regulatory mechanism involving Wnt expression and signaling.
~62 kDa observed
Immunogen
Histidine-tagged recombinant protein corresponding to human GPR177.
Application
Anti-GPR177 Antibody, clone YJ5 is an antibody against GPR177 for use in WB & IC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GPR177 in HeLa cells (Prof. D.M. Virshup, Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School.) (Also reference Coombs, G.S., et al. (2010). J Cell Sci. 123(19):3357-3367).
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Wenhui Wang et al.
Neoplasia (New York, N.Y.), 18(12), 711-723 (2016-11-17)
Aberrant activation of Wnt/β-catenin signaling plays a key role in the onset and development of hepatocellular carcinomas (HCC), with about half of them acquiring mutations in either CTNNB1 or AXIN1. However, it remains unclear whether these mutations impose sufficient β-catenin
Induction and transport of Wnt 5a during macrophage-induced malignant invasion is mediated by two types of extracellular vesicles.
Menck, K; Klemm, F; Gross, JC; Pukrop, T; Wenzel, D; Binder, C
Oncotarget null
Leandro S D'Abronzo et al.
American journal of clinical and experimental urology, 10(5), 299-310 (2022-11-01)
Resistance to androgen receptor (AR) targeted therapies remains as the main reason for most prostate cancer related deaths. Lineage plasticity resulting in altered, treatment insensitive prostate tumor cell phenotypes such neuroendocrine differentiated prostate cancer is a common manifestation within resistant
Jonathan Stewart et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(3), 428-436 (2014-09-27)
The oncogenic role of WNT is well characterized. Wntless (WLS) (also known as GPR177, or Evi), a key modulator of WNT protein secretion, was recently found to be highly overexpressed in malignant astrocytomas. We hypothesized that this molecule may be
Alan P Lombard et al.
American journal of clinical and experimental urology, 7(4), 203-214 (2019-09-13)
De-regulation of Wnt signaling pathways has been shown to be associated with progression of castration-resistant prostate cancer and more recently, studies indicate that both canonical and non-canonical Wnt pathways may mediate resistance to anti-androgen therapies such as enzalutamide. However, the
Contenu apparenté
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MABS87 | 04053252686450 |
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