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Merck

NA03

Anti-PCNA (Ab-1) Mouse mAb (PC10)

liquid, clone PC10, Calbiochem®

Synonyme(s) :

Anti-Proliferating Cell Nuclear Antigen

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203

Principaux documents

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Nom du produit

Anti-PCNA (Ab-1) Mouse mAb (PC10), liquid, clone PC10, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

PC10, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, yeast, mouse, human, zebrafish

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (2.5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (not recommended with unconjugated antibody)
Paraffin Sections (2.5 µg/mL, no pre-treatment required))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PCNA(5111)
zebrafish ... Pcna(30678)

Analysis Note

Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue

Application

Immunoblotting (2.5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (not recommended with unconjugated antibody, see comments)

Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

Immunogen

Epitope: Within amino acids 112-121
recombinant PCNA

Other Notes

Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.

Packaging

Please refer to vial label for lot-specific concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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