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Merck

03117332001

Roche

Bovine Serum Albumin Fraction V, protease-free

from bovine serum

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About This Item

NACRES:
NA.21
UNSPSC Code:
12352202

Nom du produit

Bovine Serum Albumin Fraction V, protease-free, from bovine serum

biological source

bovine

assay

≥98.5% albumin basis (electrophoresis)

form

lyophilized

packaging

pkg of 50 g

manufacturer/tradename

Roche

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Analysis Note

Contaminants: ≤0.001% heavy metals; proteases not detectable (casein digest)

Application

Bovine Serum Albumin Fraction V, protease-free is used:
  • for stabilization of purified enzymes
  • for site-blocking reagent in ELISA techniques
  • as a protein standard for determination of protein concentration

Biochem/physiol Actions

Bovine Serum Albumin (BSA) facilitates the transmission of drugs, hormones, and fatty acids. It is the most commonly used blocking agent in enzyme-linked immunosorbent assay (ELISA). BSA enhances the differentiation of human embryonic stem cells (hESC) and is also a chief component of cell culture media.

General description

Bovine serum albumin (BSA) is a globular, α-helical, non-glycosylated protein is produced in the liver. It consists of three homologous, structurally different domains and two sub-domains each. BSA has 17 cysteine residues cross-linked and bound into a single chain.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Glennys V Reynoso et al.
Methods in molecular biology (Clifton, N.J.), 2023, 287-299 (2019-06-27)
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.
D J Speca et al.
Genes, brain, and behavior, 13(4), 394-408 (2014-02-06)
The Kv2.1 delayed rectifier potassium channel exhibits high-level expression in both principal and inhibitory neurons throughout the central nervous system, including prominent expression in hippocampal neurons. Studies of in vitro preparations suggest that Kv2.1 is a key yet conditional regulator
Drew C Tilley et al.
The Journal of general physiology, 151(3), 292-315 (2018-11-07)
Allosteric ligands modulate protein activity by altering the energy landscape of conformational space in ligand-protein complexes. Here we investigate how ligand binding to a K+ channel's voltage sensor allosterically modulates opening of its K+-conductive pore. The tarantula venom peptide guangxitoxin-1E
Amanda B Hummon et al.
BioTechniques, 42(4), 467-470 (2007-05-11)
A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic aberrations and their
Myra E Woodworth-Hobbs et al.
The Journal of nutritional biochemistry, 25(8), 868-874 (2014-05-20)
Saturated fatty acids like palmitate contribute to muscle atrophy in a number of conditions (e.g., type II diabetes) by altering insulin signaling. Akt is a key modulator of protein balance that inhibits the FoxO transcription factors (e.g., FoxO3) which selectively

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