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Roche

Adenosine Deaminase (ADA)

from calf intestine

Synonyme(s) :

ADA, Adenosine Deaminase

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A propos de cet article

UNSPSC Code:
12352204
Numéro CE :
3.5.4.4 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
Biological source:
bovine intestine
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biological source

bovine intestine

form

suspension

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

packaging

pkg of 1 mL (10 mg)

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-7.4

solubility

water: miscible

suitability

suitable for chemiluminescence

NCBI accession no.

AAF91430

UniProt accession no.

P56658

application(s)

life science and biopharma

foreign activity

AMP-deaminase <0.01%, AP <0.01% (with pre incubation), Guanase <0.01%, NP <0.01%

storage temp.

2-8°C

Gene Information

bovine ... ADA(280712)

General description

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.
Chemiluminescent adenosine assay.

Biochem/physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Analysis Note

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
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