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UNSPSC Code:
12352207
MDL number:
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Laissez-nous vous aiderNom du produit
Fibronectine, from human plasma
biological source
human plasma
sterility
non-sterile; 0.2 μm filtered
assay
95% (SDS-PAGE)
form
lyophilized (clear, colorless solution after reconstitution)
mol wt
440 kDa
packaging
pkg of 5 mg
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
human ... FN1(2335)
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Disclaimer
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Application
Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)
Biochem/physiol Actions
Active on most mammalian cells
General description
Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Physical form
Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.
Preparation Note
Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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