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Merck

11442074001

Roche

BM-Purple

Roche, pkg of 100 mL, solution

Synonyme(s) :

chromogenic substrate

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A propos de cet article

UNSPSC Code:
12352204
NACRES:
NA.54
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Nom du produit

BM-Purple, Roche, pkg of 100 mL, solution

form

solution

packaging

pkg of 100 mL

manufacturer/tradename

Roche

color

dark purple

solubility

H2O: insoluble

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Application

  • Colorimetric AP substrate for: Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

General description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

Other Notes

For life science research only. Not for use in diagnostic procedures.
Ready-to-use solution.

Preparation Note

Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Consulter la Bibliothèque de documents

Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
The cell differentiation of the musculoskeletal system is highly coordinated during limb development. In the distal-most region of the limb, WNT and FGF released from the apical ectodermal ridge maintain mesenchymal cells in the undifferentiated stage. Once the cells stop
Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Jonas Malkmus et al.
Nature communications, 12(1), 5557-5557 (2021-09-23)
Precise cis-regulatory control of gene expression is essential for normal embryogenesis and tissue development. The BMP antagonist Gremlin1 (Grem1) is a key node in the signalling system that coordinately controls limb bud development. Here, we use mouse reverse genetics to
Deepika Sharma et al.
eLife, 10 (2021-10-15)
Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we
Chunyan Li et al.
Nature communications, 12(1), 1094-1094 (2021-02-19)
Seahorses have a circum-global distribution in tropical to temperate coastal waters. Yet, seahorses show many adaptations for a sedentary, cryptic lifestyle: they require specific habitats, such as seagrass, kelp or coral reefs, lack pelvic and caudal fins, and give birth

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