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UNSPSC Code:
41116158
NACRES:
NA.54
Storage temp.:
2-8°C
Shipped in:
wet ice
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sufficient for ≤1,000 tests
manufacturer/tradename
Roche
technique(s)
ELISA: suitable
detection method
chemiluminescent
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Catégories apparentées
General description
Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Application
Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:
- for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
- to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
- to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines
The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
- Detection and quantification of cell proliferation induced by growth factors and cytokines
- Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
- Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
- Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Biochem/physiol Actions
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.
Packaging
1 kit containing 7 components.
Other Notes
- BrdU Labeling Reagent, 1,000x conc
- FixDenat
- Anti-BrdU-POD 4. Antibody Dilution Solution
- Washing Buffer PBS, 10x conc.
- Substrate Component A
- Substrate Component B
For life science research only. Not for use in diagnostic procedures.
Composants de kit seuls
Réf. du produit
Description
- BrdU Labeling Reagent
- FixDenat ready-to-use
- Anti-BrdU-peroxidase antibody
- Antibody Dilution Solution ready-to-use
- ready-to-use 10x concentrated
- Substrate Component A
- Substrate Component B
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1
Classe de stockage
3 - Flammable liquids
wgk
WGK 1
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