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Merck

11814389001

Roche

Red Blood Cell Lysis Buffer

solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Synonyme(s) :

RBC Lysis Buffer, lysis buffer, red blood cells

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UNSPSC Code:
23201100
NACRES:
NA.54
Service technique
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form

solution

usage

sufficient for 50-500 reactions

packaging

pkg of 100 mL

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for further applications. This buffer is not intended for use with whole blood from any other species.

Application

Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for use in further research applications.
It has been used for the lysis of blood cells in femoral bone marrow, PBMC (peripheral blood mononuclear cells) and human lung adenocarcinoma samples.
Red blood cell lysis buffer has been used in the isolation of PBMCs (peripheral blood mononuclear cells) from HIV infected patients. It has also been used in the isolation of lung cellsm, naive CD8+ T cells and infected cells from the lung.

Physical form

Ready-to-use solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.


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flash_point_f

No data available

flash_point_c

No data available



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Dual Specificity Phosphatase 5 Is Essential for T Cell Survival
Kutty RG, et al.
PLoS ONE, 11(12), 0167246-0167246 (2016)
Quantification of membrane transporter proteins in human lung and immortalized cell lines using targeted quantitative proteomic analysis by isotope dilution nanoLC?MS/MS
Fallon JK, et al.
Journal of Pharmaceutical and Biomedical Analysis, 154, 150-157 (2018)
Jeroen J A van Kampen et al.
PloS one, 5(7), e11409-e11409 (2010-07-14)
HIV protease inhibitors must penetrate into cells to exert their action. Differences in the intracellular pharmacokinetics of these drugs may explain why some patients fail on therapy or suffer from drug toxicity. Yet, there is no information available on the