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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.54
Conjugate:
unconjugated
Clone:
monoclonal
Application:
—
Citations:
17
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Laissez-nous vous aiderbiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
isotype
IgG1
storage temp.
2-8°C
Catégories apparentées
General description
Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.
Application
Anti-His6 is used for the detection of His6-tagged proteins in:
- Immunoblotting (e.g., dot blots and western blots)
- Immunoprecipitation
- Immunoassays (ELISA)
- Immunocytochemistry
- Immunofluorescence
Biochem/physiol Actions
Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
Preparation Note
Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
- Immunoprecipitation: 0.5 to 5 μg/ml
- Western blot: 0.2 to 0.5 μg/ml
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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