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Merck

114413

Bromothymol Blue

ACS reagent, Dye content 95 %

Synonyme(s) :

3′,3″-Dibromothymolsulfonphthalein

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A propos de cet article

Formule empirique (notation de Hill) :
C27H28Br2O5S
Numéro CAS:
76-59-5
Poids moléculaire :
624.38
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847171
EC Number:
200-971-2
Beilstein/REAXYS Number:
373934
MDL number:
MFCD00005872

Principaux documents

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InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

grade

ACS reagent

form

powder

composition

Dye content, 95%

color

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C ((392 - 396 °F) - lit.), 200-202 °C (lit.)

λmax

420 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol, ≥8000 at 326-332 nm in methanol, ≥9000 at 279-285 nm in methanol

storage temp.

room temp

Quality Level

200

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Catégories apparentées

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000352454
0000352443
0000352454
0000352443
0000293123

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Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Mariana A Sanchez et al.
Analytica chimica acta, 694(1-2), 95-99 (2011-05-14)
Liquid-liquid microextraction without phase segmentation was implemented in a multicommuted flow system for determination of the anti-hypertensive diltiazem. The procedure was based on ion pair formation between the drug and the dye bromothymol blue at pH 3.5. The detection was
K R Klein et al.
Journal of clinical microbiology, 47(11), 3669-3672 (2009-10-02)
Cryptococcus neoformans and Cryptococcus gattii are closely related pathogenic fungi. Cryptococcus neoformans is ecologically widespread and affects primarily immunocompromised patients, while C. gattii is traditionally found in tropical climates and has been reported to cause disease in immunocompetent patients. l-Canavanine
Bambang Kuswandi et al.
Talanta, 74(4), 613-618 (2008-03-29)
An optical fiber biosensor consisting of acetylcholinesterase (AChE) and bromothymol blue (BTB) doped sol-gel film was employed to detect organophosphate pesticide chlorpyrifos. The main advantage of this optical biosensor is the use of a single sol-gel film with immobilized AChE
Sevgi Tatar Ulu
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 67(3-4), 778-783 (2006-10-13)
A simple, rapid, accurate, precise and sensitive colorimetric method for the determination of finasteride in tablets is described. The proposed methods are based on the formation of ion-pair complexes between the examined drug with bromophenol blue (BPB), bromocresol green (BCG)
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