Accéder au contenu
Merck

61135

Violet cristallisé

suitable for microscopy

Synonyme(s) :

Chlorure d’hexaméthylpararosaniline, Violet 3 de base, Violet de gentiane, Violet de méthyle 10B

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

Formule linéaire :
C25H30N3Cl
Numéro CAS:
548-62-9
Poids moléculaire :
407.98
PubChem Substance ID:
329759365
UNSPSC Code:
12171500
NACRES:
MA.02
MDL number:
MFCD00011750
Colour Index Number:
42555
Beilstein/REAXYS Number:
3580948

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Violet cristallisé, suitable for microscopy

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

form

powder

technique(s)

microbe id | staining: suitable

color

green to very dark green

visual transition interval

0.1-2.0, yellow-green to blue-violet

mp

205 °C (dec.) (lit.)

density

1.190 g/cm3

ε (extinction coefficient)

≥1750 at 585-595  nm in water

suitability

suitable for microscopy (Bact., Bot., Hist., Vit.)
suitable for microscopy

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

Quality Level

200

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

  • Crystal violet is mainly used in Gram staining and its variants, and for staining amyloid, bacterial components, and vascular plant tissues.
  • It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
  • It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

Biochem/physiol Actions

Gram staining is used to differentiate bacteria based on their cell wall structure. Gram-positive bacteria have a thicker cell wall with more peptidoglycan and less lipid content, while gram-negative bacteria have a thinner cell wall with less peptidoglycan and more lipid content. During the staining process, crystal violet is applied to the bacteria, followed by the iodine to form a complex that fixes the dye. A decolorizer is then used to remove the dye from gram-negative bacteria by dissolving their lipid layer. Gram-positive bacteria retain the dye due to the dehydration and shrinking of their cell walls. A counterstain, such as safranin or basic fuchsin, is applied to impart a pink color to decolorized gram-negative bacteria.
The antimicrobial properties of crystal violet are due to free radical generation and the formation of unionized dye-bacteria complexes. It inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

General description

Crystal violet, also known as Basic Violet 3 or Gentian Violet, is a basic, cationic aminoarylmethane dye. It is the N-hexamethylated derivative of pararosaniline and bears a lipophilic cation. It is primarily used in bacteriology and microscopy as the primary stain in Gram staining to classify and distinguish between Gram-positive and Gram-negative bacteria. It is indicator grade, exhibiting a color transition from yellow-green in acidic conditions to blue-violet in basic conditions. It also possesses antibacterial and antifungal properties.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCN2851
BCCL8536
BCCK6571
BCCK9044
BCCH3825

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
Histological and Histochemical Methods: Theory and Practice
J.A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Klaus Hunger
Industrial Dyes: Chemistry, Properties, Applications, 47-47 (2007)
Weixiao Liu et al.
Journal of industrial microbiology & biotechnology, 31(3), 127-132 (2004-04-08)
One laccase-secreting engineered strain and four white-rot fungi were tested for their capacity to decolorize nine dyes that could be classified as azo, anthraquinonic and triphenylmethane dyes. Trametes versicolor was the most efficient of the tested strains under these experimental
Tomoyuki Fujita et al.
Journal of the National Cancer Institute, 100(13), 940-949 (2008-06-26)
Neuroblastomas are characterized by hemizygous 1p deletions, suggesting that a tumor suppressor gene resides in this region. We previously mapped the smallest region of consistent deletion to a 2-Mb region of 1p36.31 that encodes 23 genes. Based on mutation analysis
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique