70191
Mueller Hinton Agar
NutriSelect® Plus, according to FDA, NCCLS, powder, for bacteria, pack of 500 g, pack of 100 g, pack of 2.5 kg
Synonyme(s) :
MH agar; Antibiotic sensitivity test media; disc diffusion test media
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A propos de cet article
NACRES:
NA.74
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Agency:
NCCLS
Form:
powder
Suitability:
bacteria, nonselective for Neisseria spp., nonselective for bacteria (General Media)
Application(s):
clinical testing
food and beverages
microbiology
food and beverages
microbiology
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Laissez-nous vous aideragency
NCCLS
reg. compliance
according to FDA
sterility
non-sterile
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 17.0 g/L , beef infusion solids, 2.0 g/L , casein hydrolysate, 17.5 g/L , starch, 1.5 g/L
packaging
pack of 100 g, pack of 2.5 kg, pack of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | susceptibility testing: suitable, microbiological culture: suitable
final pH
7.3±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
storage temp.
10-30°C
suitability
bacteria, nonselective for Neisseria spp., nonselective for bacteria (General Media)
Quality Level
Catégories apparentées
Application
Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.
General description
Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:
- It shows good reproducibility from batch to batch
- It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
- It supports the growth of both fastidious and non-fastidious bacterial pathogens
- The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
- It is a loose agar and therefore facilitates better diffusion of antibiotics
- In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
- In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
- In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli
Other Notes
Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Preparation Note
Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Preparation and evaluation of QbD based fusidic acid loaded in situ gel formulations for burn wound treatment
Aksu NB, et al.
Journal of drug delivery science and technology, 52, 110-121 (2019)
Solid-state structure and antimicrobial and cytotoxicity studies of a cucurbit [6] uril-like Cu6L4 constructed from 3, 5-bis [(1H-tetrazol-5-yl) methyl]-4H-1, 2, 4-triazol-4-amine
Vasylevskyi S, et al.
Acta Crystallographica Section C, Structural Chemistry, 74(11) (2018)
R D Jenkins et al.
Journal of clinical microbiology, 22(3), 369-374 (1985-09-01)
Disk diffusion susceptibility tests for enterococci are frequently modified by adding 5% sheep blood (SB) to Mueller-Hinton agar; the performance standards from the National Committee for Clinical Laboratory Standards sanction this addition. Susceptibility testing of aminoglycoside antibiotics is not recommended
Mehmet Evren Okur et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 28(3), 338-348 (2020-03-21)
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and
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