Accéder au contenu
Merck

85927

ECC ChromoSelect Selective Agar

NutriSelect® Plus, suitable for microbiology

Synonyme(s) :

E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
41171606
NACRES:
NA.85
MDL number:
MFCD01634645

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

ECC ChromoSelect Selective Agar, NutriSelect® Plus, suitable for microbiology

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 10.0 g/L
casein enzymic hydrolysate, 3.3 g/L
chromogenic mixture, 0.43 g/L
disodium hydrogen phosphate, 1 g/L
peptone, special, 6.0 g/L
sodium chloride, 2 g/L
sodium dihydrogen phosphate, 0.6 g/L
sodium pyruvate, 1 g/L
sorbitol, 1 g/L
Tergitol™
7, 0.15 g/L
tryptophan, 1 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

Quality Level

200

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol™ inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCN2022
BCCL3003
BCCH4374
BCCG1479
BCCB8207

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hongda Wang et al.
Light, science & applications, 9, 118-118 (2020-07-21)
Early identification of pathogenic bacteria in food, water, and bodily fluids is very important and yet challenging, owing to sample complexities and large sample volumes that need to be rapidly screened. Existing screening methods based on plate counting or molecular

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique