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Merck

89640

Toluidine Blue

suitable for microscopy, 8.74% (ZN (THEORY))

Synonyme(s) :

Tolonium chloride; Basic Blue 17

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A propos de cet article

Formule empirique (notation de Hill) :
C15H16ClN3S · 0.5ZnCl2
Numéro CAS:
6586-04-5
Poids moléculaire :
373.97
PubChem Substance ID:
329769757
UNSPSC Code:
12171500
NACRES:
MA.02
MDL number:
MFCD03548293
Colour Index Number:
52040
Beilstein/REAXYS Number:
4068052

Principaux documents

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Nom du produit

Toluidine Blue, suitable for microscopy, 8.74% (ZN (THEORY))

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C

InChI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

InChI key

DAQXTSXITPUGRG-UHFFFAOYSA-L

assay

8.74% (ZN (THEORY))

form

powder or crystals

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

εmax

≥800 at 621-636 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Catégories apparentées

Application

Toluidine Blue is suitable for use in the following applications:
  1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
  2. in metachromatic staining of sulfated mucins and related materials
  3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
  4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Biochem/physiol Actions

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

General description

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCK0876
BCCL0360
BCCH1582
BCCD9721
BCBZ9485

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Stephen H Schlecht et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(9), 1910-1919 (2019-05-02)
Postnatal development and the physiological loading response of the anterior cruciate ligament (ACL) complex (ACL proper, entheses, and bony morphology) is not well understood. We tested whether the ACL-complex of two inbred mouse strains that collectively encompass the musculoskeletal variation
Newman Osafo et al.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 30(8), 732-744 (2019-08-17)
Xylopic acid (XA) has been reported to exhibit analgesic activity, alleviate neuropathic pain in rodents, and demonstrate anti-inflammatory effects. Intrarectal challenge of rats with acetic acid induces colitis that bears resemblance in terms of its pathogenesis, histopathology, and inflammatory profile
Ulrike Kettenberger et al.
Journal of tissue engineering and regenerative medicine, 11(7), 1974-1985 (2015-11-10)
Locally applied bisphosphonates, such as zoledronate, have been shown in several studies to inhibit peri-implant bone resorption and recently to enhance peri-implant bone formation. Studies have also demonstrated positive effects of hydroxyapatite (HA) particles on peri-implant bone regeneration and an
Yamuhanmode Alike et al.
Neural regeneration research, 14(12), 2173-2182 (2019-08-10)
The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research has shown that the accuracy of axon
Xiaoli Guo et al.
PLoS pathogens, 13(2), e1006142-e1006142 (2017-02-06)
Stem cell pools in the SAM (shoot apical meristem), RAM (root apical meristem) and vascular procambium/cambium are regulated by CLE-receptor kinase-WOX signaling modules. Previous data showed that cyst nematode CLE-like effector proteins delivered into host cells through a stylet, act
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