Kanamycine sulfate from Streptomyces kanamyceticus

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic purified from the bacterium Streptomyces kanamyceticus. Kanamycin Sulfate is a selection agent for cells in culture media and acts by binding to the 30S subunit of the bacterial ribosome which inhibits protein synthesis in susceptible bacteria.

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used: in the research of cell type-specific expression of genes

Mode d'action : Ce produit agit en se fixant à la sous-unité ribosomale 70S, ce qui inhibe la translocation et provoque des erreurs de codage.

Mode de résistance : les enzymes modifiant les aminoglycosides (dont l'acétyltransférase, la phosphotransférase et la nucléotidyltransférase) peuvent modifier cet antibiotique, l'empêchant alors d'interagir avec les ribosomes.

Spectre antimicrobien : Le sulfate de kanamycine est efficace sur les bactéries à Gram négatif, les bactéries à Gram positif et les mycoplasmes.

Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

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