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Merck

T3163

Tetramethylrhodamine isothiocyanate Isomer R

powder

Synonyme(s) :

6-TRITC, Tetramethylrhodamine-6-isothiocyanate

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A propos de cet article

Formule empirique (notation de Hill) :
C25H21N3O3S
Numéro CAS:
80724-20-5
Poids moléculaire :
443.52
NACRES:
NA.47
PubChem Substance ID:
24900112
UNSPSC Code:
12171500
MDL number:
MFCD00063517

Principaux documents

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Nom du produit

Tetramethylrhodamine isothiocyanate Isomer R, powder

InChI

1S/C25H21N3O3S/c1-27(2)16-6-9-19-22(12-16)31-23-13-17(28(3)4)7-10-20(23)24(19)21-11-15(26-14-32)5-8-18(21)25(29)30/h5-13H,1-4H3

SMILES string

CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4cc(ccc4C([O-])=O)N=C=S)=[N+](/C)C)c1

InChI key

OBYNJKLOYWCXEP-UHFFFAOYSA-N

description

labeling efficiency with bovine albumin >= 50%

form

powder

composition

Dye content, ≥80%

solubility

1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

Catégories apparentées

Application

  • TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry.
  • Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands.
  • TRITC-antibodies are used to identify pathogenic amoebae.
  • TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms.
  • TRITC-Iabeled reagents have also been used as fluorescent probes of live cells.
  • Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior.

General description

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

hcodes

H412

pcodes

P273

Hazard Classifications

Aquatic Chronic 3

Classe de stockage

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000434860
0000434860
0000343008
0000301239
0000343008

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

In Vitro Study of Nuclear Assembly and Nuclear
Import Using Xenopus Egg Extracts
Douglass J Forbes
Methods in Molecular Biology (2016)
E. Harlow and D. Lane, ed.
Antibodies: A Laboratory Manual, 353-355 (1988)
Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
Yanyan Jia et al.
Neuron, 105(2), 310-321 (2019-11-26)
Transmembrane channel-like (TMC) 1 and 2 are required for the mechanotransduction of mouse inner ear hair cells and localize to the site of mechanotransduction in mouse hair cell stereocilia. However, it remains unclear whether TMC1 and TMC2 are indeed ion
Thomas E Wood et al.
Cell reports, 29(1), 187-201 (2019-10-03)
The type VI secretion system (T6SS) is crucial in interbacterial competition and is a virulence determinant of many Gram-negative bacteria. Several T6SS effectors are covalently fused to secreted T6SS structural components such as the VgrG spike for delivery into target
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