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T4375

Tetrazolium Blue Chloride

used in colorimetric determination of reducing compounds

Synonyme(s) :

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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A propos de cet article

Formule empirique (notation de Hill) :
C40H32Cl2N8O2
Numéro CAS:
1871-22-3
Poids moléculaire :
727.64
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24900207
EC Number:
217-488-8
Beilstein/REAXYS Number:
3894077
MDL number:
MFCD00040933
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Quality Level

200

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

Application

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
WXBF5235V
WXBF3911V
WXBF0836V
WXBD9808V
WXBF1353V

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Consulter la Bibliothèque de documents

Preparation of a new tetrazolium salt which yields a blue pigment on reduction and its use in the demonstration of enzymes in normal and neoplastic tissues.
A M RUTENBURG et al.
Cancer research, 10(2), 113-121 (1950-02-01)
C K Jue et al.
Journal of biochemical and biophysical methods, 11(2-3), 109-115 (1985-08-01)
Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as
Ternary complex of molybdenum(VI) with 4-nitrocatechol and tetrazolium blue chloride and Its application to extraction-spectrophotometric analysis of ferrous metallurgy products.
Dimitrov., A.N., et al.
Journal of Analytical Chemistry, 62, 122-125 (2007)
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