72464
Atto 565 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
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A propos de cet article
Formule empirique (notation de Hill) :
C35H34ClN3O11
Poids moléculaire :
708.11
UNSPSC Code:
12352108
NACRES:
NA.32
MDL number:
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BioReagent
assay
≥90% (HPLC), ≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
Quality Level
Catégories apparentées
General description
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Features and Benefits
- Strong absorption
- High fluorescence quantum yield
- High thermal and photo-stability
Other Notes
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Michael Cooper et al.
Journal of fluorescence, 14(2), 145-150 (2004-12-24)
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an
Benjamin Zoller et al.
Cell, 175(3), 835-847 (2018-10-20)
How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent transcriptional activity in early Drosophila embryos and characterize the variability in absolute activity levels across expression boundaries.
Elizabeth A Jares-Erijman et al.
Nature biotechnology, 21(11), 1387-1395 (2003-11-05)
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of
Rachael Bakker et al.
eLife, 9 (2020-06-23)
Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the
Matthew G Niepielko et al.
Current biology : CB, 28(12), 1872-1881 (2018-06-05)
The formation of ribonucleoprotein assemblies called germ granules is a conserved feature of germline development. In Drosophila, germ granules form at the posterior of the oocyte in a specialized cytoplasm called the germ plasm, which specifies germline fate during embryogenesis. mRNAs
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