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Merck

85558

Spermidine

BioUltra, Molecular Biology, ≥99.5% (GC)

Synonyme(s) :

SPD, 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

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A propos de cet article

Formule linéaire :
NH2(CH2)3NH(CH2)4NH2
Numéro CAS:
Poids moléculaire :
145.25
UNSPSC Code:
12352116
NACRES:
NA.26
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
Assay:
≥99.5% (GC)
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InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

SMILES string

NCCCCNCCCN

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (GC)

storage condition

(Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.)

technique(s)

ChIP: suitable, buffer exchange: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.479 (lit.)

pH

12.0-13.5 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

0.925 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.05

suitability

suitable for molecular biology

application(s)

advanced drug delivery
genomic analysis

Quality Level

Gene Information

rat ... Ppm1a(24666)

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General description

Spermidine is a polyamine and a trivalent organic cation.Spermidine is a naturally occurring polyamine compound with diverse biochemical properties. It plays a vital role in gene expression, participating in DNA replication, transcription, and translation processes. Spermidine is crucial for cell growth, proliferation, and differentiation, influencing cell cycle progression and tissue development. It possesses antioxidant activity, safeguarding cells against oxidative stress by scavenging free radicals. Spermidine also stimulates autophagy, promoting the turnover of damaged organelles and protein aggregates for cellular homeostasis. With neuroprotective effects, it supports neuronal survival, synaptic plasticity, and guards against neurodegenerative diseases. Additionally, spermidine modulates cellular signaling pathways involved in inflammation and immune responses.

Application

Spermidine has been used:
  • as a component of chromosome isolation buffer
  • as a component of transcription buffer
  • as a component of homogenization buffer

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Spermidine modulates various cellular activities like cellular growth and development, stability of DNA and apoptosis.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Molecular Biology and Biochemical research
  • Free from DNase, RNase, NICKase, and protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for trace levels of Anions and Cations

Analysis Note

liquid or solid

Other Notes

For additional information on our range of Biochemicals, please complete this form.
In first strand cDNA synthesis; Effect on endonuclease inhibition by agarose contaminants

Disclaimer

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Classe de stockage

8A - Combustible corrosive hazardous materials

flash_point_f

233.6 °F - closed cup

flash_point_c

112 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension for analysis of repetitive DNA by flow cytometry
Brind AJ and Lansdorp PM
Protocol Exchange (2011)
The effect of spermidine on endonuclease inhibition by agarose contaminants.
J P Bouché
Analytical biochemistry, 115(1), 42-45 (1981-07-15)
Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue
Cutler AA, et al.
Bio-protocol, 7(24) (2017)
Restriction Endonucleases and Modification Methylases
Enzymology Primer for Recombinant DNA Technology, 233-306 (1996)
Induction of Autophagy and Apoptosis in Melanoma Treated With Palladacycle Complexes
Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging, 231-247 (2016)

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